摘要
目的 探讨pcEgr IFNγ基因 放射联合治疗对移植黑色素瘤B16小鼠的抗肿瘤作用及其免疫学机理。方法 肿瘤局部注射脂质体包裹的pcEgr IFNγ重组质粒后 36h ,接受 2 0GyX射线照射 ,观察放疗后不同时间肿瘤大小 ,放疗后 3d瘤内IFNγ转录水平和 15d部分免疫学指标。结果pcEgr IFNγ基因 放射联合治疗后第 3～ 15天 ,肿瘤生长速率明显低于单纯照射组 ,照后第 9～ 15天明显低于单纯基因治疗组和空质粒对照组 ;治疗后第 3天 ,pcEgr IFNγ基因 放射联合治疗组瘤内IFNγ表达水平明显高于单纯基因治疗组 ;脾脏NK细胞毒活性、IL 2和IFNγ分泌活性均明显高于单纯放疗组和单纯基因治疗组。结论 pcEgr IFNγ基因 放射联合治疗抗肿瘤作用明显优于单纯放疗和单纯基因治疗组 ,其机理可能与放疗诱导瘤内IFNγ基因表达增强 ,激活荷瘤机体抗肿瘤免疫功能有关。
Objective\ To study the anti\|tumor effect of IFN γ gene\|radiotherapy to murine melanoma and its immunologic mechanism. Methods\ pcEgr\|IFNγ plasmids were injected locally into tumor,and 36 hours later,the tumors were given 20 Gy X\|ray irradiation.Tumor growth at different time,IFN γ expression 3 days later and immunologic indexes 15 days later were detected. Results\ At 3-15 days after pcEgr\|IFNγ gene\|radiotherapy,the tumor growth rate was lower than that of irradiation alone group.It was also lower than that of gene therapy alone group and control plasmid combined with X\|ray irradiation group significantly. Day 3 tumor IFN γ expression was higher than that of plasmid treatment alone group.NK activity,IL\|2 and IFN γ secretion activities were higher than those of genetherapy alone and irradiation alone groups significantly. Conclusion\ The anti\|tumor effect of IFN γ gene\|radiotherapy is better than that of either of them applied solely.Its mechanism might be concerned with the higher expression of IFN γ induced by irradiation in tumors and activation of anti\|tumor immunologic functions.\;
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
2001年第4期236-238,共3页
Chinese Journal of Radiological Medicine and Protection
基金
国家自然科学基金资助项目 (3 9970 2 2 9)
