摘要
目的 :研究中药方剂提取物A2 与肿瘤多药耐药之间的关系。方法 :建立多药耐药细胞系SPCA1/ADM ,通过MTT法测定细胞耐药性及耐药性的逆转作用 ,用流式细胞技术检测P -糖蛋白 (P -glycoprotein ,P -gp)的表达 ,用荧光法测定细胞内ADM的积累。结果 :A2 本身具有抑癌作用 ,且其无或低细胞毒浓度 ( 5 0 μg/ml、10 0 μg/ml)可增加SPCA1/ADM对阿霉素的敏感性 2 9.5倍及 40 .5倍。 10 0 μg/mlA2 +异搏定 (Verapamil ,VLP) 10 μg/ml ,使SPCA1/ADM的敏感性由 2 9.5倍增至 45 .5倍。提示两者有协同作用。A2可抑制P -gp的表达 ,并明显增加SPCA1/ADM细胞内阿霉素含量。结论 :SPCA1/ADM具有多药耐药表型。中药A2 可能通过抑制P -gp功能 。
Objective:To observe the relations between the extract A 2 from compound Chinese herbs and multidrug resistance(MDR)of cancer.Methods:establishing SPCA 1/ADM cells with MDR and determining MDR and the reversal effect,P-gp expressions and intensity of adriamycin in the cells by MTT assay,floucytometry and fluorescence assessment respectively.Results:A 2 itself has effects of restraining cancer and adding sensitivity of SPCA 1/ADM cell to ADM with its low or no toxic concentration(50μg/ml,100μg/ml)by 29.5 and 40.5 times,and increasing sensitivity of SPCA 1/ADM with Verapamil(VLP)from 29.5 to 45.5 times,which suggesting A 2 and VLP both have synergis.P-gp expressions of SPCA 1/ADM cells may disappeared with A 2 and adriamycin was significantly increased.Conclusions:The SPCA 1/ADM cells had MDR phenotypes.A 2 can be related to depression of the P-gp expression whereby an increase of the ADM accumulation in the cells.
出处
《中医药研究》
2001年第4期40-43,共4页
Research of Traditional Chinese Medicine
基金
江苏省科技计划项目 (批准号 :BJ2 0 0 0 31 1 )
