摘要
目的 应用PCR扩增法检测耐甲氧西林葡萄球菌(MRS)的mecA基因。方法 临床分离的 161株葡萄球菌 ,应用PCR扩增法鉴定MRS的mecA基因 ,并与Oxacillin纸片扩散法进行比较。结果 161株葡萄球菌用Oxacillin纸片扩散法和mecA基因法检测MRS有 4株菌有差异 ,mecA基因法鉴定阴性而Oxacillin纸片扩散法鉴定为耐药株 ,3株纸片扩散临界耐药 ,mecA基因法鉴定阳性 ,两种方法的符合率是96 89%。结论 mecA基因检测技术可以准确、快速判定MRS ,特别是在鉴定纸片扩散法临界耐药株具有优势。
Objective To establish a method of polymerose chain reaction(PCR) for identification of clinical staphylococcal isolates.Methods A total of 161 clinical staphylococcal isolates were tested to identify mecA gene in Methicillin-Resistant Staphylococcus(MRS) by PCR and Oxiacillin disk diffusion method.Results Among the 161 staphylococcal isolates,there were four strains negative for mecA gene by PCR,while resistant by disk diffusion.Three of 161 MRS strains showed borderline resistance.All three strains were tested positive for mecA by PCR.The consistence of two methods was 96.89%.Conclusion The technology of PCR to rapid identification of MRS was superior to disk diffusion method especially for borderline-resistant strains.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2001年第9期847-848,共2页
Chinese Journal of Public Health
