摘要
目的探讨用逆转录病毒载体转入 m IL - 3基因的基质细胞系 QXMSC1对骨髓造血细胞前体的影响。方法用含鼠 IL - 3基因的逆转录病毒载体感染骨髓基质细胞系 QXMSC1,获得 IL - 3高表达细胞系 QXMSC1IL - 3用于实验。观察QXMSC1IL - 3细胞上清对骨髓前体细胞 CFU- GM、CFU - E、CFU - GEMM的影响 ,QXMSC1IL - 3基质细胞对长期培养起始细胞的影响。结果 QXMSC1IL- 3培养上清对骨髓前体细胞、CFU- GM、CFU- E、CFU- GEMM有明显促进作用。QXMSC1IL- 3能明显增加有核细胞数和长期培养起始细胞数 ,诱导分化形成 CFU- GM增多。阻断基质细胞与造血细胞相互接触 ,QXMSC1IL- 3促造血细胞增殖作用有所减弱。结论基质细胞 QXMSC1IL- 3可能作为有效的基因载体进一步促进骨髓移植后或辐射损伤后的造血和免疫功能重建。
ObjectiveTo observe whether bone marrow stromal cell line QXMSC1 engineered to secrete IL-3 (QXMS1 IL-3) can accelerate the hematopoietic function in vitro.MethodsQXMAC1 IL-3 cell was constructed with transduiced mIL-3 using retrovirus vector and the highest secreting cells was used in the following expriments.Short-term culture was used to observe CFU-GM,CFU-E?CFU-GEMM in adding QXMSC1 IL-3 supernatant.Longterm culture-initiating cells (LTC-IC) was measured and then separated from the stromal layer by micro-porous membrane to observe hematopoietic progenitors in the absence of stromal cells QXMSC1 IL-3.ResultsQXMSC1 IL-3 significantly increased the number of CFU-GM,CFU-E and CFU-GEMM in short-term culture as compared with QXMSC1 and also improved the production of granulocyte-macrophage progenitors in long term culture.Though the absence of direct contact between hematopoietic function and stromal QXMSC1 IL-3,the number of nucleated cells and CFU-GM decreased,still significantly increased than that of QXMSC1 and QXMSC1 plxsn.ConclusionThese data demonstrated that the bone marrow stromal cell line QXMSC transduced with IL-3 (QXMSC1 IL-3) can improved the hematopoietic function in vitro and may be used in cell therapy and gene therapy in animal models.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2001年第4期285-288,共4页
Immunological Journal
