摘要
目的 :探讨检测胆汁中p5 3基因突变对胆囊癌的诊断价值。方法 :采用PCR SSCP银染法 ,检测 15例胆囊癌及 10例良性胆囊疾病胆汁中p5 3基因的突变情况 ,并对胆汁标本进行细胞学检查。结果 :胆汁细胞学检查诊断胆囊癌的阳性率为 13% ,特异性为 10 0 %。 15例胆囊癌胆汁中有 9例检出p5 3基因突变 ,占6 0 % ,其中 8例细胞学检查为阴性 ;10例良性胆汁均未检出p5 3基因突变。结论 :检测胆汁中p5 3基因突变 ,具有高度的特异性 。
Objective:To investigate the value of detection of point mutations in the p53 gene in bile for diagnosis of gallbladder cancer(GBC).Methods:Genetic alteration of the p53 gene was examined in bile specimens of 15 GBC cases and 10 cases of benign gallbladder disease using PCR(polymerase chain reaction) SSCP(single strand conformation polymorphism) combined with silver staining. Cytologic diagnosis was performed with all of bile specimens.Results:Sensitivity and specificity of bile cytologic diagnosis for GBC were 13% and 100% respectively. The p53 gene mutation was detected in nine of the 15 GBC cases examined(60%), and not in all of 10 benign bile specimens. The DNA analysis demonstrated the presence of p53 gene mutations in 8 cases of GBC with false negative cytologic diagnosis.Conclusion:Detection of point mutations in the p53 gene in bile is highly specific for diagnosing GBC and may be a valuable diagnostic modality for GBC.
出处
《肝胆胰外科杂志》
CAS
2001年第2期76-78,共3页
Journal of Hepatopancreatobiliary Surgery
基金
南京市卫生局科研资助项目! (NO .JY9811)
