摘要
目的 :研究CPC对BMP的体外缓释作用及其药代动力学 ,为将CPC与BMP制成复合型生物活性人工骨提供理论依据。方法 :以单纯CPC为对照组 ,以按 1gCPC固相配 5mgrhBMP -2的配比将CPC与rhBMP -2复合作为实验组 ,浸入 0 .0 1mol/L的磷酸盐缓冲液中 ,分别在第 1、2、3、4、7、14、30、90、180、36 0d取缓释液 ,用高效液相色谱仪分析不同时间rhBMP -2缓释量 ,绘制rhBMP -2缓释曲线 ,总结药代动力学规律。结果 :第 1～ 7drhBMP -2出现快速释放 ,释放曲线呈快速上升直线 ;第 7～ 14drhBMP -2释放处于平台期 ;第 14～ 36 0drhBMP -2出现缓慢而持久的释放 ,释放符合Higuchi方程。结论 :CPC能对rhBMP -2有缓慢而持久的体外缓释作用 ,有望充当rhBMP -2的缓释载体 。
Objective:In order to study CPC's sustained release of rhBMP-2 and its kinetics in vitro.Methods:We immerged the CPC/rhBMP-2 compound(1g CPC powder to 5 mg rhBMP-2)into 0.01 mol/L phosphate buffer solution.The solution including rhBMP-2 released from CPC/rhBMP-2 compound was extracted after 1,2,3,4,7,14,30,90,180 and 360 days respectively.The extracted solution was analyzed by Liquid Chromatograph.The release profile of rhBMP-2 from CPC was scaled and its kinetics was assayed.Results:rhBMP-2 had been released rapidly during the 1st to 7th day,slowly and lastingly during the 14th to 360th day,and almost at a platform period during the 7th to 14th day.The release of rhBMP-2 from CPC/rhBMP-2 compound during the 14th to 360th days followed the Higuchi model equation.Conclusion:It can be concluded that CPC showed its characteristics as a sustained delivery system for rhBMP-2 and may be used as a novel sustained release carrier for rhBMP-2 in clinic.
出处
《中国矫形外科杂志》
CAS
CSCD
2001年第8期783-786,共4页
Orthopedic Journal of China
基金
国家自然科学基金资助项目 (项目批准号 39970 748)
关键词
磷酸钙水泥
骨形态发生蛋白
缓释系统
药代动力学
Calcium Phosphate Cement(CPC)
Bone Morphogenetics Protein
Sustained release system
Pharmaco kinetics
