摘要
目的 :探讨蛛网膜下腔出血 (SAH)后脑血管痉挛 (CVS)时脑组织一氧化氮 (NO)和内皮素 1(ET 1)含量变化及尼莫地平 (ND)对其影响。方法 :将 135只Wistar大鼠随机均分为SAH组、ND处理组和假手术组 ,观察手术前后基底动脉管径 ,及 2 4h内局部脑血流量 (rCBF)、脑组织NO和ET 1含量动态改变 ,并行海马病理检查。结果 :SAH后rCBF明显而持续降低 ,基底动脉管径显著缩小 ;海马CAl区锥体细胞严重受损 ;脑组织NO和ET 1含量均在SAH后1～ 2 4h显著增加 (P <0 .0 5～ 0 .0 1)。ND处理后使上述异常变化均减轻。结论 :SAH后脑组织NO、ET 1增多可能参与了CVS所致脑损害过程 ,ND通过减轻CVS和拮抗脑组织NO及ET
Aim: To investigate the changes of nitric oxide and endothelin 1 levels in brain tissue in rat with cerebral vasospasm (CVS) following subarachnoid hemorrhage(SAH), and the protective effect of nimodipine. Methods: Wistar rats were divided randomly into SAH group, nimodipine treated group and sham operated group. Diameters of basilar artery before and after operation were measured, and dynamic changes of regional cerebral blood flow (rCBF), brain nitric oxide and endothelin 1 levels were determined within 24 h.Pathological examination of hippocampus was also performed.Results:SAH resulted in significant and persistent decrease of rCBF. Diameter of basilar artery was reduced markedly. Neurons in hippocampus CA1 subfield were damaged severely. Both nitric oxide and endothelin 1 levels in brain tissue were increased significantly from 1 h to 24 h after SAH. However, the pathological changes of above parameters were not so severe in nimodipine treated rats. Conclusion: Increase of nitric oxide and endothelin 1 levels in brain tissue is probably involved in the development of ischemic brain damage caused by CVS after SAH. Nimodipine exerts its protective effect on brain neurons by antagonizing CVS and the pathological changes of nitric oxide and endothelin 1 in brain tissue.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2001年第3期255-258,共4页
Chinese Journal of Applied Physiology
