摘要
目的 利用伯氏疏螺旋体基因工程抗原外膜蛋白C(OspC)建立间接ELISA ,检测莱姆病特异性抗体IgM。方法 基因工程抗原OspC的包被浓度和酶标抗 μ链单抗所用浓度及血清稀释倍数 ,均由方阵滴定法确定 ,并进行精密度、特异性试验、阻断试验和干扰试验。结果 OspC最佳浓度为 15 0 μg/L ,批内平均变异系数 4 6 % ,批间平均变异系数 14 2 % ,用ELISA测定临床已确诊莱姆病 33例 ,5 7例正常体检者 ,同时与进口ELISA试剂盒比较 ,两方法符合率 97 8%。结论 该方法特异性强、敏感性高、实验结果可靠 ,是莱姆病早期诊断的好方法。
Objective\ To detect specific IgM of Lyme disease indirect ELISA using recombinant outer surface protein C(OpsC)of Borrelia burgdorferi in vitro was established. Methods \ Coated concentration of recombinant OspC and dilution multiple of serum anol concentration of enzyme secondary antibody were determined by block,and degree of percision.specificity interference and interruption test were performed. Results\ Best concentration of OspC was 150 μg/L.within run CV was 6.3% between run CV was 11.8%.Confimred 33 Lyme patients in clinic and 57 controls were examined meanwhile by this method and import ELISA kit,coincidena rate was 97.8%.Conclusion\ This ELISA using recombinant OspC was a good method for early diagnostic of Lyme disease.
出处
《临床检验杂志》
CAS
CSCD
北大核心
2001年第3期159-160,共2页
Chinese Journal of Clinical Laboratory Science
