摘要
目的 :探讨补肾复方用于破骨细胞药效观察的含药血清制备条件之一 (量效关系 )。方法 :取体重 2 70± 2 0 g的 SD大鼠 ,雌雄各半 ,每组 10只 ,同等条件下制备补肾复方含药血清和对照血清 ,分别观察不同灌胃剂量和血清添加量的含药血清对骨吸收陷窝数量的影响。结果 :随着灌胃剂量和含药血清添加量的提高 ,其骨吸收陷窝数明显减少。结论 :补肾复方含药血清对破骨细胞骨吸收功能具有明显的抑制作用 ;补肾复方用于破骨细胞药效观察的大鼠含药血清制备条件之一为等效剂量连续 2次 (间隔 2 h)灌胃、末次灌胃后 1h采血 ,其血清添加浓度为 30 %。
Object To evaluate the condition of drug sera on osteoclast(dose effect relationship).Method SD rats, average weight 270±20g, half female and half male, each group has 10 rats. On the same condition collect the effect Bushen Fang sera and control sera, observe of different dosage and different sera addition on the change in value of resorption lucuna.Result The more dosage and more sera addition, the lower the lucuna number. Conclusion Bushen Fang sera could inhibit osteoclast resorption, and the condition was two administrations(between 2h),1h after the second administration collecting sera, the sera addition was 30%(V/V).
出处
《浙江中医学院学报》
2001年第3期16-17,共2页
Journal of Zhejiang College of Traditional Chinese Medicine
基金
浙江省自然科学基金!资助项目 ( 3990 10 )
国家九五攻关项目! ( 96 90 6 0 90 5 )
关键词
补肾复方
含药血清
量效
破骨细胞
吸收陷窝
Bushen Fang,drug sera,dose effect relationship,osteoclast,resorption lacuna
