摘要
目的 建立酒石酸唑吡坦血药浓度测定方法。方法 采用HPLC 荧光检测法测定血药浓度 ,激发波长 2 5 4nm ,发射波长390nm。血样加 0 .2 5mol·L-1KOH碱化后用乙醚萃取 ;色谱柱 :HypersilODS2 (2 0 0mm× 4.6mm ,5 μm) ;流动相 :乙腈 0 .0 2mol·L-1KH2 PO4 (pH6 .0 ) (4 0∶6 0 ) ;流速 :1.0mL·min-1。结果 血药浓度线性范围 5 .0～ 2 5 0 .0ng·mL-1(r=0 .9995 ,n =6 ) ,血浆最低检测浓度为 2 .5ng·mL-1,高、中、低 3种浓度平均回收率分别为 :(10 3.6 0± 2 .44 ) % ,(10 4.40± 0 .84) % ,(10 6 .6 4± 9.93) %。
OBJECTIVE: To establish a method for determining zolpidem tartrate in human plasma. METHODS: A high-performance liquid chromatographic assay with fluorimetric detection was developed, the excitation and emission wavelengths were 254 nm and 390 nm, respectively. The plasma sample was extracted with diethyl ether after alkalization by addition of 0.25 mol·L-1 KOH solution. The sample was separated on Hypersil ODS2 column (200 mm × 4.6 mm, 5 μm), with CH3CN-0.02 mol·L-1KH2PO4(pH6.0)(40:60) as the mobile phase (flow rate at 1.0 mL·min-1). RESULTS: The calibration curve was linear over the range of 5.0 [similar to] 250 ng·mL-1(r = 0.9995, n = 6). The limit of detection was 2.5 ng·mL-1. The mean recoveries of high, medium and low concentrations were (103.60 ± 2.44)%, (104.40 ± 0.84)%, (106.64 ± 9.93)%, respectively. CONCLUSION: This study provided a reliable quantitative method for the pharmacokinetic study of zolpidem.
出处
《中国药学杂志》
EI
CAS
CSCD
北大核心
2001年第5期333-335,共3页
Chinese Pharmaceutical Journal
