摘要
目的 :通过观察前列腺素 E1(PGE1 )对缺血再灌注大鼠心肌一氧化氮 (NO)及一氧化氮合酶 (NOS)活性的影响 ,探讨 PGE1 保护心肌的机制。方法 :18只 SD大鼠随机分成 3组 ,正常组、缺血再灌注组和 PGE1 组 ,以结扎大鼠冠脉左室支 30 min后再灌注生理盐水 6 0 m in作为缺血再灌注组 ,灌注 PGE1 12 .5 μg/ kg为实验组 ,用 RT- PCR方法检测心尖心肌组织 e NOS m RNA和 i NOS m RNA的变化 ,通过凝胶图象分析系统对 RT- PCR产物电泳条带进行密度分析 ,硝酸还原酶法测定血浆 NO浓度。结果 :缺血再灌注后 i NOS升高 ,e NOS、NO下降 ;而 PGE1 组与缺血再灌注组比较 ,e NOS明显升高 ,i NOS变化无显著差异 ,NO上升。结论 :PGE1 通过提高 e NOS活性水平而达到对缺血再灌注心肌的保护作用。
Objective: This study observed the effects of PGE 1 on NO and NOS activity of ischemia-reperfusion myocardial and investigated the mechanism of protective effects in rats. Methods: Eighteen SD rats were divided randomly into three groups. Myocardial ischemia was induced by ligation of left anterior descending(LAD)artery for 30 min followed by 60 min reperfusion(control group).PGE 1 12.5μg/kg , within 60 min was given continously during the reperfusion period(PGE 1 group). Rats without LAD occlusion were taken as Sham group(Sham operation group).RT-PCR was used to measured the variety of NOS mRNA and radioimmunoassay was used to detected the variety of NO in blood. The density of PCR products was determined by image analysis. Results: After reperfusion, iNOS mRNA was up, eNOS mRNA and NO were down, while eNOS mRNA was up significantly and NO increased in PGE 1 group. Conclusions: PGE 1 afford an excellent protection on rats MI/R myocardial through increasing eNOS activity.
出处
《南通医学院学报》
2001年第3期237-238,241,共3页
ACTA Academiae Medicinae Nantong
