摘要
目的 :研究银杏内酯对缺氧神经细胞的保护作用。方法 :采用原代培养的小鼠大脑皮层神经细胞 ,利用MTT比色法观察了不同浓度 (终浓度为 0 .5 9～ 37.5 μg/ ml)的银杏内酯对缺氧神经细胞的作用。检测细胞外液 L DH释放量以观察 37.5μg/ ml浓度的银杏内酯对缺氧神经细胞存活的影响 ,并进行了形态学的观察。结果 :银杏内酯在所研究的浓度范围之中对神经细胞的保护作用呈现出浓度依赖性 ;并且 37.5 μg/ ml浓度的银杏内酯能明显提高缺氧神经细胞的活性和生存能力 ,减轻细胞的形态学损伤。结论 :银杏内酯对小鼠神经细胞缺氧损伤有明确的保护作用。
Objective: To determine whether ginkgolide has protective effects on hypoxic-induced neuronal damage. Methods: Various concentrations of ginkgolid(0.57~37.5μg/ml) were used to observe their effects on hypoxic cultured mouse cortical neurons and the neuronal viability in primary cultures from mouse cerebral cortex was assessed using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay . Then, the measurement of the release of LDH(lactate dehydrogenase) and morphological observation were used to research the effects of ginkgolide (37.5μg/ml) on neuronal death. Result: Ginkgolide could enhance the neuronal viability in a dose-dependent manner in the range from 0 to 37.5μg/ml ,and, ginkgolide (37.5μg/ml) enhanced the neuronal viability and decrease the cell death rate significantly. Conclusion: Ginkgolide has affirmative protective effects on hypoxia-induced neuronal damage.
出处
《南通医学院学报》
2001年第3期226-228,共3页
ACTA Academiae Medicinae Nantong
基金
交通部九.五通达计划资助项目 ( 95 -0 4-0 3-4 1)
