摘要
目的 建立降脂I号口服液的质量控制方法。方法 薄层色谱法鉴别该中成药中的大黄素、大黄酚、大黄酸和大黄素甲醚 ,薄层扫描法定量测定大黄酚。结果 色谱板为硅胶HCMC -Na ,展开剂正已烷 -乙酸乙酯 -甲酸 (30∶10∶0 .5 )上层 ,薄层扫描波长 434nm ,大黄酚检测限为 0 .0 5 μg,标准曲线Y =410 1.8X - 1919(r =1.0 0 0 ) ,平均回收率 99.77%。结论 所建立的方法特征性强 ,方法学考察满意 。
Aim To establish an analytical method for the quality control of Jiangzhiyihao.Method TLC with emodin,chrystophanol,rhein and physcion as standard compounds was adopted to identify the drug,while TLC-Scanning with chrystophanol as standard component was used to control the drug's quality.Results Silica H-CMC Na plate was used with n-Hexane-Ethylestate-Formic acid (30∶10∶0.5) as developing solvent,TLCS wavelength at 434 nm,detection limit was 0.05μg,the equation of the calibration curve was Y=4101.8X-1919 (r=1.000),the average recovery was 99.77%.Conclusion The established analytical method is characteristic for the quality and quantity of this Chinese medicine,and can be used as the routine quality control method of this drug.
出处
《解放军药学学报》
CAS
2001年第4期211-212,214,共3页
Pharmaceutical Journal of Chinese People's Liberation Army
关键词
薄层层析
大黄蒽醌类
定性
定量
降脂I号
含量
Thin-layer chromatography
Rheum Athraquinoines
Qualitation
Quantitation
Jiangzhiyihao
