摘要
根据已发表序列设计一对包含鸡传染性喉气管炎病毒 ( ILTV) TK基因全长核苷酸的 1 2 5 9bp引物 ,对 2株 IL TV强毒和1株 ILTV疫苗毒进行 PCR扩增 ,均能扩增出预期大小的目的片段 ,酶切分析证实了目的片段的特异性 ,而其它禽病原体的扩增均为阴性。 PCR检测 IL TV DNA的最小检测量为 75 pg。此方法检测人工接种鸡的气管棉拭样品 ,均能检测到 IL TV。
A pair of primers which inculded infectious laryngotracheitis virus (ILTV) TK gene was desiged and synthesized.When PCR was performed with 2 virulent ILTV , 1 vaccine ILTV and other pathogenic microorganisms,the amplified fragment of ILTV was about 1 259 bp which could be confirmed by restriction endonuclease analysis.,others were negative.75pg ILTV DNA could be detected by PCR. All of the tracheal sponge samples from experimentally infected chickens were amplified a specific 1 259 bp fragment of TK gene. The results suggested that the method can be used for detection and diagnosis of ILTV in clinic samples.
出处
《动物医学进展》
CSCD
2001年第3期59-61,共3页
Progress In Veterinary Medicine
基金
扬州市发展基金项目资助 (YZ990 75)
