摘要
目的 探讨瘢痕形成对汗腺再生的影响。 方法 分别取幼儿和成人大面积深度烧伤后瘢痕组织及其自身健康皮肤组织 ,切片后采用免疫组化方法检测角蛋白 19(K19)和角蛋白14(K14)在瘢痕组织中汗腺分泌部和导管部的表达 ,并以此确定汗腺在瘢痕组织中的定位和分布。同时 ,采用HE染色法观察正常皮肤组织中汗腺组织的分布。 结果 正常皮肤中可见完整的汗腺结构 ,包括汗腺分泌部和导管部。瘢痕组织中可见K19和K14的阳性表达信号 ,其中K19的阳性染色主要位于瘢痕基底部真皮深层与正常皮下组织交界处 ,呈团状 ,表明这些部位存在汗腺分泌部。K14的阳性染色散见于瘢痕组织中 ,呈同心圆状 ,为汗腺的导管部。 结论 严重烧伤后创面存在汗腺再生的生物学基础和潜力。在增生性瘢痕中之所以没有汗腺的重建 ,可能与瘢痕组织修复速度超过汗腺再生的速度或增生性瘢痕在创面形成一个屏障 ,阻碍了汗腺的再生有关。
Objective To explore the possible effects of scar on sweat gland regeneration after injury. Methods Keratin 19 (K19) and keratin 14 (K14) were used as the markers for the secreting part and tube part of sweat glands respectively. Biopsies of normal skin and hypertrophic scar were taken from children (4-10 years) and adults (35-51 years). Immunohistochemistry was used to observe the distributing characteristics of sweat glands in the normal skin and scars. Results The positive expression cells of K19 in scar were seen between the boundary of scar and reticular layer of the dermis, indicating that some sweat glands were located in the deep part of the scar. Whereas, K14 positive cells were seen in scar tissues, which meant that there were some circle like tubes of sweat gland. Conclusions These results indicate that there are some remaining sweat gland tissues in scar, which offer the possibility for sweat gland regeneration after injury.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
2001年第6期338-340,共3页
Chinese Journal of Trauma
基金
国家重点基础研究发展规划专项经费资助项目(G19990 5 42 0 4)
国家杰出青年科学基金资助项目 ( 395 2 5 0 2 4)
关键词
烧伤
瘢痕
汗腺
角蛋白
表皮干细胞
Burns
Cicatrix
Sweat glands
Keratin
Stem cell, epidermis
