摘要
针对芦荟自然繁殖率低下的情况 ,探讨了利用组织培养方法解决生产中繁殖的问题 .在叶和茎 2种外植体中 ,嫩茎的出愈率最高 ,是理想的快速繁殖材料 ,较适宜的诱导愈伤组织的激素组合是BA 2 .0mg·L- 1 +IBA 0 .2mg·L- 1 ,诱导不定芽的适宜激素组合是BA 3.0mg·L- 1 +NAA 1 .0mg·L- 1 ,而根的诱导则是在 1 / 2MS +NAA 1 .0mg·L- 1 +庶糖 1 .5% +活性碳 3%培养基上进行 .
Because the fruiting rate and germinative percentage of Aloe vera L. are lower, tissue culture was explored to solve the propagating problem in the production. The results show that the bud is the best material in speeding propagation among the two explants (stem, leaf) as it has the biggest inductivity, the best growth and it is easy to be inducted adventitious buds. The suitable phytohormone compositions to induct callus, adventitious buds and roots are respectively BA 2.0 mg·L -1 +IBA 0.2 mg·L -1 , BA 3.0 mg·L -1 +NAA 1.0 mg·L -1 ,1/2 MS+NAA 1.0 mg·L -1 +sugar 1.5%+carbon3%.
