摘要
以矮化鲜食枣优良品种梨枣为试材，研究了适宜其组培快繁技术的条件。试验结果表明，冬季休眠和夏季生长的１年生枣头枝均可作为外植体材料；通过改进外植体杀菌处理方法，可降低褐化率和提高成活率。试验同时筛选出适宜的启动培养基为：ＭＳ＋０．ＳＢＡ＋０．１１ＢＡ（顶芽茎段）和 Ｍ＋２．０ＢＡ＋０．１ＩＢＡ（腋芽茎段）；最佳分化和生根培养基分别为：ＭＳ＋２．０ＢＡ＋０．１ＮＡＡ、１／２ＭＳ＋１．０ＩＢＡ十０．０５ＩＡＡ＋０．１ＮＡＡ。
This paper studied on the appropriate factors in tissue culture for rapid propagation of the best jujube variety (Z. jujube Mill. Var. inermis Rehd.) 桳izao. The results show that the best mediums for initial, proliferous and rooting culture are MS + 0.5BA + 0.1 IBA (Topbud stemstick)、 MS + 2.0BA + O.lffiA (Budding stemstick), MS + 2.0BA + 0.1NAA, 1/2MS + l.OIBA + 0.05IAA + 0.1NAA respectively, and show that brown death rate of plant is reduced and percent of live tissue is raisedby improving the method of disinfecting bacterium. In addition, other valuable results have been obtained.
出处
《中国农学通报》
CSCD
2001年第4期34-36,共3页
Chinese Agricultural Science Bulletin
基金
山西省自然科学基金资助课题。
