摘要
目的 探讨检测T淋巴细胞核仁形成区 (NORs)酸性非组蛋白对肿瘤诊断和病情监测的应用价值。方法 应用普通细胞银染技术对体外激活的患者外周血淋巴细胞染色 ,通过计算机显微图像分析技术 ,计算硝酸银染色酸性非组蛋白 (Ag) NORs面积与核面积的比值 (IS % ) ,对 10 46名健康体检人员、393例非恶性疾病患者和 70 6例各种肿瘤患者进行了对比研究。结果 发现恶性疾病与健康人和非恶性疾病患者之间 ,T淋巴细胞Ag NORs活性差异有显著意义 (P <0 0 5 )。在正常人和非恶性疾病中 ,T淋巴细胞Ag NORs检测的平均IS %值分别为 (7 81± 0 6 9) %和 (7 85± 0 72 ) % ,其中IS %值大于 6 %的比例分别为 99 8%和 94 7% ,两者差异无显著意义 (P >0 0 5 )。恶性肿瘤患者IS %均值为 (5 17± 0 87) % ;不同肿瘤及肿瘤患者不同临床时期 ,IS %值小于 6 %的比例为 6 0 %～ 92 3% ;不同肿瘤之间比较 ,结直肠癌及乳腺癌与其他肿瘤之间差异有显著意义 (P <0 0 5 ) ,而其他肿瘤之间差异无显著意义。与癌胚抗原 (CEA)、甲胎蛋白 (AFP)等肿瘤标志物比较 ,T淋巴细胞Ag NORs检测具有更高的诊断符合率 (2 6～ 92 3% )。结论 T淋巴细胞Ag NORs检测 ,在肿瘤诊断。
Objective To analyze the Ag NORs in T cells of different patients to find clues for the diagnosis and monitoring of cancer.Methods A KL 2 imaging system was used to analyze the areas of Ag NORs in T cells. T cells from peripheral blood were cultivated and stimulated, and silver staining was performed. The areas of Ag NORs were analyzed.Results A total of 1 046 nomal adults, 393 patients with non malignant diseases and 706 patients with malignant diseases were analyzed. Their average IS% values were (7.81±0.69)%, (7.85±0 72)% and 5.17±0.87 respectively. The IS% values above 6% were 99.8%, 94.7% and 10% to 35% respectively. No differences were observed in different kinds of cancers except breast cancer and rectum cancer whose IS% were (5.61±0.86)% and (6.10±0.92)% respectively, 75% and 26% of their IS% were less than 6% respectively. 83% to 90% of the IS% in the patients with other cancers were below 6%. In comparison with serum CEA, AFP and the like, Ag NORs in T cells were more powerful in diagnosis and monitoring of cancers.Conclusion The value of Ag NORs of T cells was significantly lower in patients with cancers and was statistically different from that in nomal adults and patients without malignant diseases. The results demonstrated that the analysis of Ag NORs might play an important role in the diagnosis, differenciation and monitoring of cancer.
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2001年第3期151-153,共3页
Chinese Journal of Laboratory Medicine
基金
科技部财政部1999年度第三批科技型中小企业技术创新基金资助项目(99C26221100365)
