摘要
目的 从分子生物学水平上探讨香烟烟雾对 DNA的生物氧化能力。方法 以 DNA加合物 8-羟基脱氧鸟苷(8- OHd G)作为 DNA氧化损伤的生物学标志物 ,用高压液相色谱 -电化学检测法对香烟烟雾染毒后的 DNA中 8- OHd G进行定量。通过气质联用法 (GC- MS)对香烟烟雾进行有机成分分析 ,用原子吸收法 (AAS)对其进行无机元素分析。结果 香烟烟雾颗粒物和挥发性有机物可不同程度地引起 DNA碱基损伤产生 8- OHd G,并分别检出有机污染物 15 7种和 78种 ,无机元素 5种。香烟烟雾对 DNA的生物氧化能力主要是由于存在大量有机污染物 ,其中的醌类、多酚等化合物具有自氧化作用 ,在体外可产生大量的活性氧自由基 ,并在金属的催化作用下进攻 DNA的碱基 ,形成加合物 8- OHd G。结论 香烟烟雾对 DNA具有氧化能力 。
Objective On the molecular biological level to studies the biological oxidative ability of Tobacco Smoke on DNA Methods The DNA adduct 8 hydroxydeoxyguanosine (8 OHdG) was used as a biomarker of oxidative DNA damage,8 OHdG of DNA exposed to tobacco smoke was detected by HPLC EC Organic and inorganic components in tobacco smoke have been analyzed by GC MS and AAS respectively Results Both particles and VOCs could cause damage of DNA base to produce 8 OHdG in different degree It was found that there were 157 and 78 kinds of organic compounds in particles and volatile organic compounds of tobacco smoke, and 5 kinds of inorganic compounds in tobacco smoke The main reason of why tobacco smoke had biological oxidative ability on DNA was that there were a lot of organic compounds, especially quinones and polyphenols in tobacco smoke, they could produce free radicals in vitro by the autooxidation,and with the catalytic reaction of metals, the DNA adduct 8 OHdG is produced Conclusion The tobacco smoke had biological oxidative ability on DNA and direct genotoxicity
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2001年第4期203-207,共5页
Journal of Environment and Health
基金
国家教育部博士点专项基金资助课题 (No.BBA2 4 A)
