摘要
目的：探讨转化生长因子（ＴＧＦ）－β１、细胞周期蛋白（ｃｙｃｌｉｎ）Ａ表达与细胞凋亡的关系及其在正常胃粘膜、非萎缩性胃炎、萎缩性胃炎、不典型增生及胃癌演化序列中的规律和可能作用。方法：用免疫组化法检测ＴＧＦ－β１和ｃｙｃｌｉｎ Ａ的表达，用ＤＮＡ原位末端标记法（ＴＵＮＥＬ）检测细胞凋亡。结果：正常胃粘膜、非萎缩性胃炎、萎缩性胃炎、不典型增生和胃癌组织的细胞凋亡指数分别为１．７％±１．６％、４．７％±５．０％、 ５．２％±４．７％、３．０％ ±１３．５％和１．６％±２．５％，非萎缩性胃炎、萎缩性胃炎和不典型增生组织的凋亡指数明显高于胃癌（Ｐ＜０．０５）；ＴＧＦ－β１的阳性表达率分别为１０．０％（１／１０）、２５．０％（４／１６）、２５．０％（１１／４４）、６０．７％（１７／２８）和５９．５０（２５／４２），正常胃粘膜、非萎缩性胃炎和萎缩性胃炎组织的阳性率与不典型增生和胃癌组织相比有显著差异（Ｐ＜０．０５）；ｃｙｃｌｉｎ Ａ的阳性表达率分别为０、６．２％（１／１６）、２０．５％（９／４４）、４６．４％（１３／２８）和８５．７％（３６／４２），不典型增生和胃癌组织的阳性率有显著差异，但两者均显著高于正常胃粘膜、非萎缩?
Background/Aims: To investigate the relationship between the expression of transforming growth factor (TGF) β1, cyclin A and apoptosis and their changes among normal gastric mucosa, non atrophic gastritis, atrophic gastritis, dysplasia and gastric carcinoma. Methods: Immunohistochemical analysis was used to detect the expression of TGF-β1, and cyclin A. Terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) method was used to detect apoptosis. Results: In normal gastric mucosa the apoptotic index was 1.7%±1.6%. In non atrophic gastritis, atrophic gastritis and dysplasia, the apoptotic indices were 4.7%±5.0%, 5.2%±14.7% and 3.0%±3.5% respectively, significantly higher than that of gastric carcinoma (1.6%±2.5%, P< 0.05). In normal gastric mucosa, non atrophic gastritis, and atrophic gastritis, the positivity rates of TGF-β1 were 10.0% (1/10), 25.0% (4/16) and 25.0% (11/44) respectively, significantly lower than that of dysplasia (17/28, 60.7%; P<0.05) and gastric carcinoma (25/42, 59.5%; P<0.05). In normal gastric mucosa, non atrophic gastritis and atrophic gastritis, the positivity rates of cyclin A were 0, 6.2% (1/16) and 20.5% (9/44) respectively, significantly lower than that of dysplasia (13/28, 46.4%; P<0.05) and gastric carcinoma (36/42, 85.7%; P<0.05), and there was also significant difference between dysplasia and gastric carcinoma (P<0.05). Conclusions: In the evolvement of gastric carcinoma the apoptotic indices increase in non atrophic gastritis and atrophic gastritis and decrease in dysplasia and gastric carcinoma. The positivity rates of TGF-β1 and cyclin A were higher in dysplasia and gastric carcinoma than those in normal gastric mucosa, non atrophic gastritis and atrophic gastritis. The expression of TGF-β1 and cyclin A may be correlated with apoptosis and may play a role in gastric carcinogenesis..
出处
《胃肠病学》
2001年第2期97-99,106,共4页
Chinese Journal of Gastroenterology
