摘要
目的：研究幽门螺杆菌（Ｈ． ｐｙｌｏｒｉ）外膜蛋白（ＯＭＰ）加大肠杆菌不耐热内毒素（ＬＴ）经口免疫对小鼠Ｈ． ｐｙｌｏｒｉ感染的保护作用。探讨定量细菌培养在疫苗免疫效果评价中的应用。方法：从Ｈ． Ｐｙｌｏｒｉ ＮＣＴＣ１１６３７菌株制备ＯＭＰ和全菌超声粉碎抗原，ＬＴ作为粘膜免疫佐剂。通过灌胃分别以Ｈ． Ｐｙｌｏｒｉ全菌超声粉碎抗原１ｍｇ加ＬＴ １０μｇ（Ａ组）、Ｈ． Ｐｙｌｏｒｉ ＯＭＰ ０．５ ｍｇ加ＬＴ １０μｇ（Ｂ组）和生理盐水（Ｃ组）免疫小鼠（ｎ＝３０），每周１次，共４次。免疫完成后４周以Ｈ． ｐｙｌｏｒｉ Ｓｙｄｎｅｙ Ｓｔｒａｉｎ１菌株攻击小鼠１次（１×１０７ ＣＦＵ／只），攻击后４周处死小鼠，取胃分别作快速尿素酶试验、病理检查及定量细菌培养，观察Ｈ．Ｐｙｌｏｒｉ定植情况。结果： Ｃ组小鼠Ｈ．Ｐｙｌｏｒｉ定植密度为２．４０×１０７ＣＦＵ／ｇ胃组织，Ａ组和Ｂ组小鼠分别为２．０３×１０５ ＣＦＵ／ｇ和６．７６×１０５ＣＦＵ／ｇ胃组织，免疫组的定植密度明显降低。结论：口服Ｈ． ｐｙｌｏｒｉ ＯＭＰ加ＬＴ对小鼠Ｈ． Ｐｙｌｏｒｉ感染具有一定免疫保护作用，但不能完全预防感染，需进一步筛选更为有效的抗原和佐剂。定量细菌培养可以?
Background/Aims: To study the protective effect of oral immunization with Helicobacter pylori (H. pylori) outer membrane protein (OMP) plus E. colt heat-labile enterotoxin (LT) in mice and explore the value of quantitative culture in the evaluation of immune effect. Methods: H. pylori NCTC11637 strain was used to prepare OMP and whole-cell sonicate antigen, LT was used as an adjuvant. 30 mice were randomized into three groups and immunized by oral administration of H. pylori whole-cell sonicate antigen 1 mg plus LT 10μg (group A), H. pylori OMP 0.5 mg plus LT 10 μg (group B) and normal saline (group C) respectively once a week for 4 weeks. 4 weeks after the last immunization, all the mice were challenged with H. pylori Sydney Strain 1 (1×107 CFU to each mouse) and sacrificed 4 weeks after the challenge. Their stomachs were collected for rapid urease test, histologic examination and quantitative culture to observe the gastric H. pylori densities. Results: Gastric H. pylori densities of group A, B and C were 2.03×105 CFU/g, 6.76×105 CFU/g and 2.40×107 CFU/g respectively. H. pylori densities of immunized groups were significantly decreased. Conclusions: Oral immunization with H. pylori OMP plus LT afford partial protection in mice challenged with H. pylori. More effective antigens and adjuvants should be explored to achieve complete protection. Quantitative culture is a sensitive and objective method to reflect the H.pylori density and to evaluate the immune effect.
出处
《胃肠病学》
2001年第2期75-77,共3页
Chinese Journal of Gastroenterology
基金
卫生部临床学科重点项目(No.97040226)
广东省科技计划重点攻关项目(No.99M04802G)
广东省自然科学基金
关键词
幽门螺杆菌
细菌外膜蛋白质炎
肠毒素炎
免疫法
定量培养
Helicobacter pylori
Bacterial Outer Membrane Protein
Enterotoxins
Immunization
Quantitative Culture
