摘要
目的用抗JEV E蛋白的mAb 2H4筛选噬菌体15肽库，以研究JEV E蛋白模拟肽。方法用生物素标记的mAb 2H4，对噬菌体随机15肽库进行3轮筛选。经ELISA鉴定后，随机挑取10个阳性噬菌体克隆测序，并与JEV E蛋白进行同源性比较。结果筛选到的噬菌体能与mAb 2H4特异地结合，并且这种结合可被天然JEV抗原所抑制。10个阳性噬菌体克隆的氨基酸序列相同，均为RQDPQWPYANSTIAR。同源分析表明，在JEV E蛋白不同区域有两个同源性较高的序列：STXAR和WXXAXST。阳性噬菌体展示肽也能与抗天然JEV抗原的鼠血清产生特异性反应。结论筛选的该噬菌体克隆展示肽可模拟JEV E 蛋白的部分抗原性。
Aim To study the mimicry peptide of JEV E protein by screening a phage 15-mer peptide library with anti-JEV E protein mAb 2H4. Methods After three rounds biopanning, the enriched positive phage clones were identified by ELISA. 10 positive phage clones were sequenced and compared homologously with JEV E protein. Results The short peptide displayed on screened positive phage could bind specifically to mAb 2H4, and the binding could be inhibited by natural JEV Ag. Amino acid sequences of the 10 positive phage clones were consensus, that is, RQDPQWPYANSTIAR. By homology analysis, two higher homologous sequences STXAR and WXXAXST were found in different regions of JEV E protein. The peptide displayed on positive phage could react specifically with the mouse antiserum against natural JEV Ag . Conclusion This peptide displayed on positive phage may mimic partial antigenicity of JEV E protein.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2001年第4期332-334,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
全军医药卫生科研基金资助
No. 98M102
