摘要
目的:改良乳鼠心肌肉细胞培养的方法,提高心肌细胞的单细胞收获率、存活率及搏动率.方法:在Simpson培养法的基础上探讨一种新的改良法,并将两种方法进行比较.结果:单细胞收获率1.60×106个/心脏;分离、消化后即刻、第5日及第8日的细胞布活率分别平均为(76.08±25.48)%、(93.32±28.67)%及(91.89±26.94)%;第5日及第8日的细胞搏动率分别平均是(91.63±25.33)%及(90.69±20.61)%,上述各项指标均高于Simpson法.结论:用改良法培养乳鼠心肌细胞时,单细胞收获率高、细胞存活率、心肌细胞和非心肌细胞分离彻底、心肌细胞纯度高.
Objective: to improve the method of neonatal rat myocytes culture. Methods: The techniques used in our new method were based on Simpson's, and they were compared with each other. Results: From the new method, the dissociation yielded an average 1.6×106 cells per heart. The ratio of trypsin blue-negative cells were 76.08% , 93.32% and 91.89% on immediately and 5, 8 days after digestion respectively, The ratio of beat cells spontaneously were 61.63% and 90.69 % on 5 and 8 days respec-tively after the beginning of cell culture. All parameters above increased in our new method than Simpson's. Conclusion: our new improved method is better at getting more free and living myocytes from neonatal rat.
出处
《中国医药导刊》
2001年第3期225-226,共2页
Chinese Journal of Medicinal Guide
