摘要
目的 探讨抗肺癌单抗 5F11与多柔比星 (DXR)的复合物 5F11 DXR对肺癌细胞的导向治疗作用及其对一种耐药肺癌细胞系的耐药逆转作用。方法 应用戊二醛法制备 5F11 DXR ,通过集落形成试验、染料排斥试验来评价其对敏感靶细胞A2、耐药靶细胞 80 1 D和 80 1 DDXR以及非靶细胞艾氏腹水癌细胞的杀伤作用。结果 多种检测方法均显示 ,在DXR含量为 0 .4μg/ml时 ,5F11 DXR对A2、80 1 D以及 80 1 DDXR细胞的杀伤作用显著高于DXR单药 (P <0 .0 5 ) ;尤其是对敏感细胞株A2细胞 ,在更低浓度 (0 .0 4μg/ml)即可表现出此种差异。但对非靶细胞艾氏腹水癌细胞 ,两者的杀伤作用无显著性差异 (P >0 .0 5 )。结论 5F11 DXR对敏感靶细胞的杀伤作用较DXR明显增强 ,并可有效逆转耐药靶细胞对DXR的多药耐药性。两者对非靶细胞的杀伤作用无显著性差异。
Objective To evaluate the inhibition effect of immunoconjugate of doxorubicin(DXR) with a monoclonal antibody, 5F11 on human lung cancer cells and its reversal effect on resistant lung cancer cells to chemotherapeutic drug. Methods DXR was attached to 5F11 using dilute glutaraldehyde crossing.The antitumor activity of immunoconjugate, 5F11 DXR, against the sensitive antigen positive cell line, A2, drug resistant antigen positive cell lines, 801 D and 801 D DXR , and antigen negative cell line, ascite cancer cell was evaluated by human tumor cell cloning assay and dye exclusion assay. Results According to the results of various assays, comparing with single DXR, 5F11 DXR could significantly increase the cytotoxicity to A2, 801 D and 801 D DXR cell lines with a DXR concentration of 0.4?μg/ml(P<0.05), and this difference was even more distinct to A2 cell line with lower concentration of DXR (0.04?μg/ml). However, there was no remarkable difference between 5F11 DXR and single DXR in cytotoxicity to antigen negative ascite cancer cell(P>0.05). Conclusion 5F11 DXR can remarkably increase the cytotoxicity of DXR to the sensitive target cells and even effectively reverse the drug resistant cell lines to DXR. There is no significant difference between 5F11 DXR and DXR in killing antigen negative cancer cells.
出处
《中国肺癌杂志》
CAS
2001年第3期169-174,共6页
Chinese Journal of Lung Cancer
