摘要
目的 :比较分析脂质代谢紊乱患者血清脂蛋白氧化易感性以及高密度脂蛋白 (HDL)对低密度脂蛋白(L DL)氧化抑制能力。 方法 :采用密度梯度超速离心分离 45例肾衰 (CRF)、33例心肌梗死 (MIS)、33例脑梗死(CI)和 5 3例 2型糖尿病 (DM)患者的血清脂蛋白组分 (VL DL,L DL,HDL) ,用硫代巴比妥酸值 (TBARS)分析脂蛋白脂质过氧化程度 ,A2 34 nm光吸收检测脂蛋白共轭双烯含量分析脂蛋白的氧化易感性 ,并与正常人 (4 4例 )进行了比较。 结果 :所有患者组 VL DL 的 TBARS值均明显高于对照组 ,其中 MIS组增高程度最为显著 (与对照组相比 ,P<0 .0 0 1) ;MIS组和 DM组 L DL、CI组和 DM组 HDL 的 TBARS值增高 ;患者组 VL DL、L DL 的氧化延滞时间也显著缩短 ,DM组缩短最为明显 ;但各组 HDL 与对照组相比无差异 ;此外患者组 HDL 对 L DL 氧化抑制能力均明显减弱 ,CRF组和 MIS组减弱最为显著。 结论 :各种脂质代谢紊乱患者体内血浆脂蛋白均发生一定程度的氧化修饰 。
Objectives:To compare the susceptibility of serum lipoproteins to oxidation and the effects of high density lipoprotein on oxidative stress in patients with lipid turbulence. Methods:VLDL, LDL and HDL were isolated using sequential ultracentrifugation from serum of patients with chronic renal failure (CRF) ( n =45), myocardial infarction survivor (MIS) ( n =33) , cerebral infarction(CI)( n =33) ,type 2 diabetes mellitus(DM)( n =53) and normal individuals ( n =44). The degree of lipid peroxidation was estimated using the thiobarbituric acid reactive substances (TBARS) value and the susceptibilities of lipoproteins to oxidation were assessed by measuring the increased absorbance value at 234 nm due to the conjugated dinene formation. Lipid levels and lipoprotein fractions were measured using standard methods. Results: VLDL obtained from the patient groups showed significant increase in TBARS values, especially from the patients with MIS (compared with control group, P <0.001). In addition, LDL from MIS and DM groups and HDL from CI and DM groups also showed markedly increase in TBARS content. Significant decrease in lag time was observed in both VLDL and LDL fractions from the four patient groups. However, no change was found in the lag time in HDL fraction from the patient group compared with control group. In addition, HDL from the four patients exhibited significantly decreased inhibitory effects on in vitro oxidation of LDL, with the most significant decrease in HDL from CRF and MIS groups. Conclusions:The oxidative modification of lipoproteins in vivo in patients with serum lipid turbulence might be involved in the development of atherosclerosis in these patients.
出处
《医学研究生学报》
CAS
2001年第3期196-199,共4页
Journal of Medical Postgraduates
关键词
脂质紊乱
脂蛋白
氧化易感性
动脉粥样硬化
Lipoproteins
Oxidative susceptibility
Lipid turbulence
Atherosclerosis
