摘要
豆乳凝固酶产生菌Bacillussp .UV 1 0的最适产酶条件 :初始pH6 4,温度 2 6℃ ,培养时间 1 9h ,需要较大的通气量。酶的最适作用pH和温度分别为 5 8和 70℃。在最适条件下酶活力可达 1 84u/mL。pH6 0～ 7 0稳定性较好。 6 0℃下 1h残余酶活 6 0 %。Ca2 +,Fe2 +,Mg2 +,Na+对其有较强的激活作用 ,而Zn2 +,Al3+则有抑制作用。
The optimum condition of shaking-flask p roducing enzyme were the tempe rature 26℃,initial pH 6 4,fermentation period 19 hours,medium volume 15mL m e dium/300mL Flask.soymilk-clotting enzyme was obtained from ammonium sulfate p r ecipitation.The optimum temperature and pH for the soymilk-clotting activity wa s 70℃and 5 8.The enzyme was easy to lose activity in acid or alkaline circumst a nce.About 60% of the original activity remained after 1 hour at 60℃.Ca 2+ ,Fe 2+ , Mg 2+ ,Na +increased the clotting activity,whereas Zn 2+ ,Al 3+ ca use inhibition.
出处
《微生物学通报》
CAS
CSCD
北大核心
2001年第4期16-19,共4页
Microbiology China
关键词
芽孢杆菌
豆乳凝固醇
最适产酶条件
酶学性质
Bacillus sp.,Soymilk-clotting enzyme ,Fermentation conditions,Properties of enzyme
