摘要
对传统的馆藏陈旧皮张标本DNA提取方法进行了改进 ,所提DNA分子量可达 1kb ,而且具有样品用量少 (约 0 0 1g)、消化时间短 (约 1 4h)和操作步骤简单等优点。利用所提DNA ,对小熊猫等珍稀动物线粒体DNA的细胞色素b和控制区序列的部分片段进行了PCR扩增、序列测定和比较分析 ,证实所提DNA合格而无污染 ,完全可以用于珍稀动物保护遗传学研究。
An improved method for DNA extraction from old or dried skins was developed. Compared to published protocols, it allows the use of minute amount of samples(0.01*!g), and requires a much reduced digestion time. With this method, DNA with size of more than 1 kb can be obtained. A portion of mitochondrial cyt b gene and control region has been amplified from total DNA of red panda, takin and gold cat, extracted from skin samples using this method. Sequence data confirmed the authenticity of the extracted DNA, and indicate thus the value of the present method in conservation genetics study.
出处
《动物学杂志》
CAS
CSCD
北大核心
2001年第4期53-57,共5页
Chinese Journal of Zoology
基金
中国科学院"青年科学家创新小组"专项基金
国家自然科学基金项目 (No .39970 10 3
30 0 70 119)资助
