摘要
利用１５％～２５％丙烯酸胺梯度凝胶的ＳＤＳ－ＰＡＧＥ分析法可将水稻种子贮藏谷蛋白分离为３个酸性（α）亚基和３个碱性（β）亚基。通过调整两性电解质比例对现有等电点聚焦电泳分析法进行改良，可将谷蛋白酸性亚基和碱性亚基分别分划为１３和１４条多肽带。将上述两种方法结合起来的双向电泳分析法可以高清晰度地离析谷蛋白并获得单一多肽。此改良的电泳分析系统有助于确定水稻谷蛋白变异及谷蛋白的生化研究。
Rice seed storage glutelin could be separated into three acidic (a-) and. hoe basic (β-) subunit components by SDS-PAGE using 15%- 25% gradient acrylamide gel. More than 13 acidic and 14 basic bands may be distinguished with an improved iso-electric focus electrophoresis system by adjusting Ampholine ratios. Combine these two sensitive electrophoresis systems resulting in two-dimensional electrophoresis giving high resolution of glutelin leading the yield of single polypeptide. The improved electrophoresis system is helpful in identifying rice glutelin variation and for further studies of glutelin biochemistry.
基金
国家自然科学基金(30040034)
中科院择优支持回国工作基金
中科院生物科学与生物技术研究特别支持费资助&&
关键词
水稻
谷蛋白
分离
等电聚焦电泳
双向电泳
种子
rice glutelin separation
iso-electric focus (IEF)
two-dimensional electrophoresis
