摘要
目的 建立扩增幽门螺杆菌全长 cag A基因的方法并对扩增的基因进行限制性酶切片段长度多态性 (RFL P)进行初步研究。方法 采用巢式 PCR与 TD- PCR结合的方法扩增 cag A,运用 Eco RI和Hind 酶切 PCR产物。结果 2 0例标本中有 13例的目的基因片段得到了扩增并得到了相应的 EFL P指纹图谱。结论 (1)我们所建立的方法能较好地扩增 cag A全长基因。 (2 ) cag A基因含有重复序列 ,并显示RFL
Objective To set up the method to amplify the entire cagA gene and study the RFLR. Methods :Using nested PCR combined with TD PCR to amplify the gene and using EcoRI and HindⅢ to generate the RFLP fingerprinting.Results: We successfully amplified the target DNA fragment from l3 among 20 samples and got the relevant RFLP fingerprinting.Conclusion: (a) The method we set up can be used to amplify the entire cagA gene.(b) cagA gene contains repeat sequence and show apparent diversity of RFLP profile.
出处
《中国微生态学杂志》
CAS
CSCD
2001年第3期127-128,134,共3页
Chinese Journal of Microecology
基金
卫生部课题!(编号 :98-1-12 3 )
