摘要
为了解去劣疏伐对种子园群体交配系统和遗传组成的影响程度 ,采用水平淀粉凝胶电泳方法 ,对 10个等位酶位点上 ,分别用混合交配模型 (ML T)和 Biosys- I程序 ,分析了去劣疏伐前后辽宁兴城油松种子园 3个年份群体的交配系统和遗传多样性变化。种子园去劣疏伐前 (1993年 )种子试样多位点异交率为 0 .96 2 ,而疏伐后 2个年份 (1996年和 2 0 0 0年 )试样的多位点异交率明显减小 ,分别为 0 .795和 0 .80 1。疏伐后种子园的自交率有升高的趋势 ,从疏伐前的 0 .0 38到疏伐后的 0 .2 0 5和 0 .199。近交系数也有明显变化 ,疏伐前为 0 .141,疏伐后分别为 0 .341和 0 .2 39。去劣疏伐后种子园的每个位点的平均等位基因数 (A)和多态位点百分率 (P)都有不同程度的降低。本文对影响种子园交配系统和遗传多样性的因素作了探讨。
Variation in mating system and genetic diversity of seed samples collected 3 years before and 3 years after thinning were examined in a clonal seed orchard of Pinus tabulaeformis Carr.in Liaoning province.Allozyme markers with 10 loci were used in the study of genetic diversity.The multilocus outcrossing,selfing and inbreeding rates of the seed orchard before thinning in 1993 were 0.962, 0.038 and 0.141 respectively.The multilocus outcrossing rate in the thinned seed orchard in 1996 and 2000 decreased to 0.795 and 0.801 respectively.At the same time the multilocus selfing rate increased to 0.205 and 0.199 and the genetic diversity indices A and P decreased to different degrees after thinning.The results showed that thinning based on phenotype had a strong influence on mating system but a lesser effect in genetic diversity indices.Factors influencing the mating system and genetic diversity are also discussed.
出处
《植物生态学报》
CAS
CSCD
北大核心
2001年第4期483-487,共5页
Chinese Journal of Plant Ecology
基金
教育部博士点科研基金 (970 0 2 2 0 8)
关键词
油松
种子园
去劣疏伐
交配系统
遗传多样性
Pinus tabulaeformis, Seed orchard thinning, Mating system, Genetic diversity
