摘要
目的 探讨在碱性成纤维细胞生长因子 (bFGF)作用下 ,人牙周膜细胞 (PDLC)的增殖、DNA合成状况的变化。方法 以常规体外组织块细胞培养法获得PDLC ,采用MTT法和3 H TdR掺入法测定bFGF对PDLC的影响 ,实验结果A值和CPM值经方差分析。结果 各实验组与对照组之间均有显著性差异 ,bFGF能显著提高PDLC的增殖活性及DNA合成 ,其效应随bFGF浓度增大而增大 ,10 0 0ng/ml范围内未见抑制现象 ,最大效应一半的浓度约为10 0ng/ml。结论 bFGF能促进PDLC增殖及DNA合成 。
Objective To evaluate the biological effects of basic fibroblast growth factor (bFGF) on proliferation and DNA synthesis of periodontal ligament cells(PDLC).Methods PDLC was isolated from periodontal ligament explants. The effects of bFGF on PDLC were assayed by MTT and3 H TdR incorporation studies. The results were expressed as A value and CPM. Analysis of variance was used.Results bFGF could enhance the proliferation and DNA synthesis of PDLC in a dose dependent manner. No inhibition was observed within 1000ng/ml bFGF concentration. The concentration of half maximal effect was 100ng/ml.Conclusions The results suggest that bFGF may play an important role in the regeneration of periodontal tissue. [
出处
《上海口腔医学》
CAS
CSCD
2001年第2期135-137,共3页
Shanghai Journal of Stomatology
