摘要
为了考察鼻咽癌表达下调 /缺失基因 NAG1 1和 NAG1 2对鼻咽癌细胞系 HNE1生长的影响 ,构建了NAG1 1和 NAG1 2基因真核表达载体 pc DNA3.1 ( +) /NAG1 1和 pc DNA3.1 ( +) /NAG1 2 ,采用脂质体转染技术将真核重组质粒和空载体质粒分别导入 HNE1细胞 ,观察转染后 HNE1细胞生物学特性的变化 .结果显示 ,NAG1 1重表达对 HNE1细胞生长和细胞周期没有明显的影响 ,而 NAG1 2重表达对 HNE1细胞有生长抑制作用 ,与空载体转染组相比 ,倍增时间由 2 4 .1 h延长至 31 .1 h,停滞于 G0 -G1期细胞数由 51 .4 2 %增加至68.1 4 % .以上实验进一步说明鼻咽癌是多基因改变的疾病 ,NAG1 2的重表达有助于鼻咽癌恶性表型的逆转 .
To study the effect of NAG11 and NAG12 gene on NAG11- and NAG12-negative nasopharyngeal carcinoma (NPC) cell line HNE1, the pcDNA3.1(+)/NAG11 and pcDNA3.1(+)/NAG12 mammalial expressing recombination were constructed and were tranfected into HNE1 cells, then cytobiologic characterization of the transfected HNE1 cells was detected by population double time (PDT) and flow cytometric analysis. The results showed that NAG11 reexpression did not play obvious role on the cell growth of HNE1, while NAG12 reexpression had definite growth suppression on HNE1, such as a longer PDT and an accumulation of cells G1 phase. These data further demonstrated that NPC is a disease involving polygenic alteration and NAG12 gene could favor malignant phenotype reversion of NPC cells.
出处
《生命科学研究》
CAS
CSCD
2001年第2期177-183,共7页
Life Science Research
基金
"8 6 3"国家重点项目!资助 (10 2 -10 -0 1-0 5 )
"973"国家重点项目!资助 (3970 0 15 8)
国家自然科学基金!资助 (30 0 0 0 0
