摘要
目的 观察ＨＩＶ－１ＣＮ ｇｐ１２０基因与ＩＬ－２基因共表达核酸疫苗质粒ｐＧＰＩＬ－２诱导产生ＣＴＬ。方法 脂质体介导共表达中国流行株ＨＩＶ－１Ｇａｇ－ｇｐ１２０基因与ＩＬ－２基因的核酸疫苗质粒ｐＧＩＬ－２转染ＢＨＫ－２１细 胞，以间接免疫光法鉴定其表达。取ｐＧＰＩＬ－２免疫鼠脾细胞，检测ｐＧＰＩＬ－２诱导的细胞毒性Ｔ细胞杀伤活性。 结果ｐＧＰＩＬ－２可有效地诱导ＣＩＬ的产生，并可杀伤ＨＩＶ－１ＣＮ嵌合基因转染的靶细胞。结论 为进一步设计中国流行株ＨＩＶ－１核酸疫苗提供了重要实验依据。
Objective To observe the cytotoxic T lymphocyte(CTL)induced with the DNA vaccine prepared by the co-expression of HIV-1CN gp 120 and IL-2 genes. Methods The plasmid pGPIL-2 for the coexpression of HW-1 Gag-gp 120 and IL-2 genes was transfected to BHK-21 cells under the mediation of liposome, and the expressed product was identified by indirect immumofluorescence method. Murine splenocytes were immunized with the pGPIL-2, and the CFL killing activity of the mice was detected. Results The CTL killing activity to the target cells transfected with HIV-1CN chimeric gene was effectively induced by pCPIL-2. Conclusion The study provides an important basis for further developing the DNA vaccine against the current HIV-1 strain in China.
出处
《中国生物制品学杂志》
CAS
CSCD
2001年第2期81-83,共3页
Chinese Journal of Biologicals
基金
国家自然科学基金(3977066)
国家杰出青年基(39825119)资助
