摘要
目的 :建立抗人甲基转移酶单克隆抗体细胞株并建立相应的蛋白测定方法。观察脑瘤组织中O6 甲基鸟嘌呤 DNA甲基转移酶 (MGMT)表达水平与亚硝脲药物疗效的关系。方法 :采用细胞融合技术建立抗人甲基转移酶单克隆抗体细胞株 ,采用免疫组织化学染色方法 ,检测 6 0例脑瘤组织中MGMT表达水平。结果 :得到 7株稳定分泌抗人甲基转移酶单克隆抗体的杂交瘤细胞株 ,并建立了测定MGMT免疫组化方法。观察的 6 0例脑瘤组织中有 2 7例MGMT阴性 ,经亚硝脲药物治疗 ,其中 2 4例好转 ,3例复发 ;另 3例MGMT可疑阳性的患者 ,经亚硝脲药物治疗 ,其中 2例好转 ,1例复发 ;2 5例MGMT阳性患者 ,经亚硝脲药物治疗 ,5例好转 ,12例复发 ,8例死亡 ;5例MGMT为强阳性患者 ,使用亚硝脲药物治疗无效 ,全部死亡。结论 :建立的 7株稳定分泌抗人甲基转移酶单克隆抗体的杂交瘤细胞株及MGMT蛋白测定方法 ,为临床开展亚硝脲预见性化疗提供了必要手段。初步结果显示 ,脑瘤组织中MGMT表达水平与亚硝脲药物疗效及预后有关 。
Objectives:To establish hybridomas secreting monoclonal antibodies(McAbs) against O 6 methylguanine DNA methyltransferase(MGMT) and to observe the relationship between MGMT expression and clinical responses to ACNU and BCNU in human brain tumors.Methods:The hybridomas were established by cell fusion.MGMT expression in 60 glioma specimens was detected by means of immunohistochemical assay.Results: Seven hybridomas secreting McAbs against MGMT were obtained.Thirty tumor specimens had no detectable or low level of MGMT expression(Mer -), while 30 specimens had high level of MGMT expression(Mer +). The Mer - patients showed more sensitive to ACNU and BCNU than the Mer + patients.Conclusions: The high specific hybridomas secreting monoclonal antibodies(McAbs) against MGMT were established.The preliminary study indicated that MGMT negative tumors were sensitive to ACNU and BCNU, whereas MGMT positive ones were more resistant to nitrosourea drugs. [
出处
《军事医学科学院院刊》
CSCD
北大核心
2001年第2期81-84,共4页
Bulletin of the Academy of Military Medical Sciences
基金
国家自然科学基金!资助项目 (3 970 0 174)
关键词
抗人甲基转移酶单克隆抗体
制备
临床应用
脑胶质瘤
亚硝脲
治疗
methyltransferases
O 6 methylguanine DNA methyltransferase
nitrosourea
glioma
antibodies,monoclonal
