摘要
根据 2 0个不同RAPD随机引物对杨树DNA的扩增结果 ,对四甲基氯化铵 (TMACI)在杨树RAPD扩增反应中的作用进行了研究 ,发现TMACI可明显改善杨树的RAPD扩增效果 ,因此TMACI可作为一种有效成分加入杨树RAPD反应体系中。同时比较了不同浓度的TMACI对杨树RAPD扩增反应的影响 ,确定最适合杨树RAPD扩增反应的TMACI浓度为 10～ 5 0 μmol L。利用 3个具亲缘关系的杨树家系对含TMACI的RAPD反应体系的实验结果进行验证 。
According to the amplification result in poplar by 20 10\|mer arbitrary primers, the effect of tetramethyl ammonium chloride (TMACI) on RAPD amplification in poplar was studied. It revealed that the effect of TMACI on RAPD amplification is significant and which can improve the result of RAPD amplification. TMACI can be used as an effective composition for assembling the RAPD reaction. The effect of different concentration ofTMACI on RAPDamplification was also studied andthe suitable concentration(10-50 μmol/L) of TMACI used in the RAPD reaction for poplar was determined. In order to test the new reaction system, three pedigrees with clear relationship were adopted, and the result showed that the new system is reliable for genetic study.
出处
《植物资源与环境学报》
CAS
CSCD
2001年第2期11-13,共3页
Journal of Plant Resources and Environment
基金
国家自然科学基金资助项目!(39770 6 2 8)
Supported by the International Foundation for Science of Swedento DrYin!(No .D 2 997
