摘要
探讨了显微注射不同浓度的外源 DNA以及温度、培养基等对小鼠原核胚体外培养发育能力的影响。显微注射 1 .5、1 5、3 0 mg/L的衰变加速因子 ( CD55)、膜反应溶解因子 ( CD59)基因后 ,小鼠原核胚发育到囊胚的比率随 DNA质量浓度的升高而依次降低为 57.7%、3 6.6%、1 0 .4 % ,差异极显著 ( P <0 .0 1 )。注射 1 .5mg/L外源 DNA,原核胚的囊胚率 ( 57.7% )比对照组 ( 54.5% )稍有提高 ,但差异不显著 ( P >0 .0 5)。 1 .5mg/L为本试验最佳外源 DNA注射质量浓度。 3 7℃、CZB培养液或改进的 Whitten氏培养液 ,可取得较好的体外培养效果。
The effect of pronuclear microinjection with foreign DNA of different concentration and culture conditions on the development in vitro of mouse pronuclear embryos was assessed in this paper. After microinjecting 1.5 mg/L, 15 mg/L and 30 mg/L of CD55 and culturing in vitro for 120 h, the blastocyst rate reached 57.7%, 36.6% and 10.4% ,respectively. There was a great difference among treatments, but no difference between the embryos microinjected with 1.5 mg/L DNA (57.7%)and control(54.5%). Injection with 1.5 mg/L of foreign DNA was optimal. Under the condition of 37℃, using improved Whitten′s medium or CZB medium , we can get the higher blastocyst rate from pronuclear embryos culture in vitro.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2001年第3期255-257,共3页
Chinese Journal of Veterinary Science
基金
上海市自然科学基金!资助项目 ( 98ZC14 0 2 5 )
上海市农业科学院青年科学基金!资助项目
