摘要
为了分离纯化二氢蝶啶还原酶制备了海格纳明吸附剂[Higenaminecarboxylic Acid(HGCA)-Sepharose]和萘醌吸附剂[Naphthoquinone(NO)-Sepharose]。酶的纯化经过了硫酸铵沉淀以及 DEAE-Sephacel、Matrex Gel、Blue A、NQ-Sepharose 和 HGCA-Sepharose一系列柱层析步骤。二氢蝶啶还原酶的电泳图谱显示了单一的谱带,相当于分子量26000的亚基。
Higenaminecarboxylie acid(HGCA)-Sepha-rose and naphthoquinone(NQ)-Sepharose havebeen prepared for the purification of dihydro-pteridine reductase EC 1.6.99.7(DHPR)fromhuman and rat liver.The enzyme was purifiedby ammonium sulphate precipitation,sequentialcolumn chromatography on DEAE-Sephacel,Ma-trex gel blue A,NQ-Sepharose and HGCA-Sepharose.SDS-PAGE of DHPR reveals a singleband corresponding to subunit M,of 26000.
出处
《化学试剂》
CAS
CSCD
北大核心
1991年第6期343-346,共4页
Chemical Reagents
基金
国家自然科学基金课题
