摘要
目的 获得旋毛虫成虫期特异性基因序列。方法 应用旋毛虫成虫期特异性探针对成虫cDNA文库进行杂交筛选、克隆测序及核苷酸序列数据库 (GenBank)查寻比较 ,采用DNAstar软件进行基因序列分析。结果 利用消减杂交 (subtractedhybridization)获得的旋毛虫成虫期特异性探针筛选成虫cDNA文库 ,获得一个长 16 2 9bp的基因。其开放阅读框架由 146 4个核苷酸组成 ,编码 487个氨基酸。序列分析表明 ,该序列是一个尚未见报道的旋毛虫基因序列 ,在 2 0 7～ 2 70位氨基酸间存在两个锌指结构 ,在 5 2～ 6 4,10 8～ 116 ,137～ 16 3,2 2 6～ 2 6 0位氨基酸处存在预测的抗原表位。结论 获得旋毛虫成虫期特异性基因 。
Objective To obtain the sequence of the adult stage specific gene of Trichinella spiralis . Methods An adult T. spiralis cDNA library was screened using an adult T. spiralis stage specific probe. The gene sequence was analyzed by DNAstar software and GenBank database. Results A cDNA clone of 1 629 bp was obtained by screening the library with an adult stage specific probe. The clone contained one open reading frame composed of 1 464 bp encoding 487 amino acids. GenBank searching revealed that no identical cDNA was found. Two zinc fingers located at the positions within amino acids 207-270. Some possible antigen epitopes were predicted within the amino acids 52-64,108-116,137-163 and 226-260. Conclusion A novel stage specific gene of adult T. spiralis encoding the protein containing certain predicted antigen epitopes was obtained.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2001年第2期68-71,共4页
Chinese Journal of Parasitology and Parasitic Diseases
基金
美国中华医学基金会 (CMB)资助项目 (Parasitology 98 6 74)&&
关键词
旋毛虫
成虫
CDNA文库
CDNA克隆
序列分析
消减杂交
Trichinella spiralis, adult cDNA library, cDNA clone, sequence analysis subtracted hybridization
