摘要
链霉菌高拷贝质粒pIJ101上的两个片段(1.18kb和0.54kb)在大肠杆菌中具有明显的启动子活性。把这两个片段克隆到链霉菌启动子探针载体pIJ424和pIJ425(修饰了克隆位点)上,转化到变铅青链霉菌中,然后用梯度平板测定转化子对卡那霉素的抗性水平发现:在变铅青链霉菌中,1.18kb的片段在两个方向都有启动子活性,而0.54kb的片段则明显没有。
Two fragments (1. 18 kb and 0. 54 kb) of the multicopy Streptomyces plasmid pIJ101 having apparent promoter activity, in E. coli were subcloned into Streptomyces promoter—probe vectors pIJ424 and pIJ425 after a modification of their cloning sites, and introduced into S. lividans by transformation. The kanamycin resistance level of these transformants showed that 1. 18 kb fragment has promoter activity in both orientations, whereas 0. 54 kb fragment has no detectable activity.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
1991年第2期180-186,共7页
Journal of Huazhong Agricultural University
