摘要
目的了解普通轮状病毒(RV)腹泻患儿病毒血症出现情况。方法应用套式一逆转录PCR方法检测60例RV腹 泻患儿外周血血浆和其中14例血清酶学异常患儿外用血单个核细胞中的RV。结果60例RV腹泻患儿中,共检出RV 病毒血症阳性者4例(6.67%),其中血浆中检出1例,外周血单个核细胞中检出3例,分属4个患儿。结论在普通RV 腹泻患儿也存在RV病毒血症。
Objective To investigate the occurrence of viremia in infants with rotavirus diarrhea. Method The rotavirus genome in the plasma samples of 60 infants with rotavirus diarrhea and in the peripherial blood mononuclear cells (PBMCs) of 14 out of the infants who showed abnormal serum enzyme levels were subjected to nest reverse transcriptase polymerase chain reaction test. Results One infant was found to be positive for rotavirus in the plasma and 3 in the mononuclear cells, and the latter 3 infants was negative for rotavirus in the PBMCs. Conclusion Rotavirus viremia may occur in infants with rotavirus diarrhea.
出处
《第一军医大学学报》
CSCD
北大核心
2001年第4期288-290,共3页
Journal of First Military Medical University