摘要
对徐长卿组织培养快速繁殖技术的试验研究结果表明，ＮＡＡ诱导愈伤组织效果优于ＩＡＡ。而ＩＡＡ较适于诱导丛生芽，ＢＡ浓度有较明显的协同作用。因此，在组织培养中激素和生长素的合理配合是达到最佳培养效果的关键。试验结果表明：诱导愈伤组织的最适培养基为Ｍｓ＋ＮＡ１．０ｇ／Ｌ＋ＮＡＡ１．０ｍｇ／Ｌ，诱导丛生芽的最适基为Ｂｓ＋ＢＡ２．０ｍｇ／Ｌ＋ＩＡＡ０．５ｍｇ／Ｌ，而诱导生根的最适培养基为１／２Ｍｓ＋ＮＡＡ１．０ｍｇ／Ｌ．通过三阶段组织培养技术的优化，可以快速繁殖出大批种苗，有效地解决徐长卿药材繁殖速度慢的问题，这一技术可望应用于生产。
series of experiments on inducing callus, developing
buds , seedlings and rcoting were done in tissuecult ure of Cynanchum paniculatum Kitag.The
results indicated that NAA was more effective in inducing callus than IAA , but IAA had better
ef-fects on inducing buds and seedling than NAA , while BA had significant cooperative rofe
both in induc-ing callus and bud. The suitable concentration of auxin and phytohormone was
the key to obtain thebest results in tissue culture. The optimized media compcosition for
inducing callus was MS+BA 1mg/L,NAA 1.mg/L. The best me-dia composition for developing
buds and seedlings was B5+BA 2 mg/L,IAA 0.5 mg/L. The satisfac-tory rooting result of vitro
plantlets was observed on the media of 1/ 2 MS+NAA 1 mg/L. This optimized three-stage
culture method was proved to be an effective way to solve the problem ofmass and
rapid-propagation in Chinese medicinal plant-Cynanchum paniculatum Kitag.
出处
《药物生物技术》
CAS
CSCD
1995年第4期33-36,共4页
Pharmaceutical Biotechnology
关键词
徐长卿
组织培养
快速繁殖
中药
Cynanchum paniculatum Kitag
Tissue culture
Rapid-propagation
