摘要
采用气相色谱法和气相色谱/质谱联用法对不同温度驯养的草鱼和鲮鱼肌肉线粒体膜脂肪酸组成进行了分析。10℃适应的草鱼和25℃适应的草鱼相比,肌肉线粒体膜脂肪酸中不饱和脂肪酸/饱和脂肪酸(U/S)值明显升高,脂酰链平均长度也从25℃时的19.08个碳原子增加到10℃时的20.46个碳原子。而鲮鱼的脂肪酸组成在不同适应温度下的变化不明显。此外,还测出了不同驯养温度下草鱼和鲮鱼肌肉线粒体中胆固醇含量的变化,发现两种鱼的胆固醇含量在低温下都显著下降。作者认为,低温适应下的草鱼由于肌肉线粒体的脂肪酸组成和胆固醇含量都发生变化,因而其肌肉线粒体膜的流动性明显增加;而鲮鱼肌肉组织线粒体中胆固醇含量虽也减少,但脂肪酸组成变化甚微,因此,鲮鱼肌肉组织线粒体膜流动性增加很有限。这可能就是鲮鱼耐寒能力差的重要原因之一。
The GC/MS and GC techniques were applied to analyse the composition of fatty acids from mitochondria in muscle tissue of grass carps and mud carps acclimated to different temperatures. Significant changes in fatty acid composition were observed in musclar mitochondrial membrane of grass carps acclimated to 25℃ and 10℃ respectively, but little changes occurred in mud carp mitochondrial membrane. The cholesterol content in musclar mitochondrial membrane of warm-and cold-acclimated fish were also determined, and the cholesterol content in all of them decreased after cold-acclimation. The fluidity of mitochondrial membranes in muscle tissue of cold-acclimated grass carps increased as a result of the changes in fatty acids and the decrease in cholesterol content. In the muscle tissues of cold-acclimated mud carps, increase in fluidity of the mitochondrial membranes was limited because the changes in fatty acid composition were little. It is believed that the difference in fluidity changes of the membrane between the grass carp and mud carp is partly responsible for the difference of cold tolerance capacity between these two fish species.
出处
《华南农业大学学报》
CAS
CSCD
1991年第A11期45-49,共5页
Journal of South China Agricultural University
关键词
草鱼
鲮鱼
脂肪酸
胆固醇
温度
Grass carp
Mud carp
Fatty acid
Cholesterol
Fluidity of membranes
Acclimation temperature
