摘要
目的应用聚合酶链反应（ＰＣＲ）检测技术，对临床可疑为单纯疱疹性角膜炎（ＨＳＫ）进行早期、快速诊断。方法用棉签擦拭病变处角膜上皮细胞，经离心、沉淀、裂解后作模板；选用Ｐｒｏｍｅｇａ公司提供的ＰＣＲ检测人单纯疱疹病毒（ＨＳＶ）－Ⅰ、Ⅱ试剂盒，设计３条引物，扩增片段分别为４６９ｂｐ和３９１ｂｐ，采用聚合酶链反应检测技术，检测其单纯疱疹病毒特异性ＤＮＡ片段。结果观察组共扩增３２例，３６眼，阳性率为９３．７５％，对照组扩增２８例２８眼，阳性率仅为２．８％。结论ＰＣＲ检测单纯疱疹病毒性角膜炎快速、简便。
ObjectiveHerpes simplex keratitis(HSK) was rapidly diagnosed by polymerase chain reaction(PCR).Methods Pathologic corneal epithelial cells taken by applicatols of cotton were isolated,precipitated and disintegrated.The isolated mRNA was made into CPNA and served as a template.The test kit of PCR for human herpes simplex virus(HSV)l,ll was used.The amplified segments of specific DNA segments of HSV are 469 bp and 391 bp,respectively.Results 93.75% of 36 eyes(32 cases) were positive in HSIC group,but only 2.8% of 28 eyes(28 cases) in control group were positive.ConclusionBy using the PCR kit to detect HSK infection,diagnosis is rapid,simple and highly specific.
出处
《眼科研究》
CSCD
1998年第2期108-109,共2页
Chinese Ophthalmic Research
