摘要
对患弧菌病的养殖斑节对虾作了细菌学分析。取典型症状的濒死对虾的血淋巴和肝胰腺组织进行细菌分离、纯化、保种，并将分离菌进行回接感染，发现有很强的致病作用，且产生与自然发病相似的症状。从感染弧菌病的病虾体内进行细菌的分离、纯化和保种，经检验发现，分离菌和回接感染后的再分离菌均为革兰氏阴性短杆菌，极生单鞭毛，有动力，对O／l 29弧菌抑制剂敏感，发酵葡萄糖产酸不产气，氧化酶和过氧化氢酶反应呈阳性，不利用乳糖、蔗糖、蜜二糖，能利用柠檬酸盐、甘露醇、果糖和半乳糖；在无盐的蛋白胨水和10％NaCl的蛋白胨水中不生长，在3％，6％，8％NaCl的蛋白胨水中生长良好，吲哚反应呈阳性，V－P反应呈阴性，赖氨酸脱羧酶、鸟氨酸脱羧酶、明胶酶和淀粉酶试验呈阳性，β－半乳糖苷酶、色氨酸脱氨酶、苯丙氨酸脱氨酶和精氨酸双水解酶反应呈阴性，可还原硝酸盐。经鉴定，该病原菌为副溶血弧菌Vibrio parahaemolyticus 。
The paper deals with the morphology and biochemical characteristics of patho-genic bacteria that are found in infected shrimps in the intensive cu1ture pond. The bacte-ria are isolated and purified from haemolymph and hepatopancreas of the infectedshrimps, and the pathogenicity of isolated bacteria and the same infected symptoms arefound after the isolated bacteria infect shrimps. Also the bacteria are isolated and puri-fied from haemolymph and hepatopancreas of the re--infected shrimps. The isolated andre--iso1ated bacteria in this study all are gram negative, short rods with a single polar fla-gellum, resistant to O/129, oxidase and catalase positive, salt requirement, growing wellin 3 %, 6 %,8 % NaCl and on TCBS, but no growth in l0% NaCl. The bacterium can uti-lize citrate, mannitol, fructose, galactose. They don't ferment lactose, inositol, sorbitol,xylose and melibiose. V--P reaction, sulfide production, arginine dighdrolase and tryptopl-can deaminase of this bacterium is negative. Bacteriological identification shows the iso-lated bacterium is Vibrio parahaemolyticus.
出处
《热带海洋学报》
CAS
CSCD
北大核心
2001年第2期80-87,共8页
Journal of Tropical Oceanography
基金
"九五"中国科学院重大项目(Z2951-B1-111)
广东省自然科学基金重点项目(963029)资助
