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分子生物学方法在医院感染监控中的应用和评价 被引量:2

The Use of Molecular Techniques in the Control of Nosocomial Infections
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摘要 简要说明了一些医院感染监控的特征和微生物学的分型方法 ,重点阐述了应用在医院感染监控和流行病学调查的分子生物学方法。这些分子生物学方法主要包括两类 :1蛋白质检测技术 ,如同工酶电泳、免疫印迹技术。 2 DNA检测技术 ,如质粒分析、染色体 DNA的限制性核酸内切酶技术、核糖分型、脉冲电场凝胶电泳和 PCR技术。最后列举了一些分子生物学方法在流行病学调查和医院感染监控中的应用。 This article reviews some aspects related to the control of nosocomial infections and organism typing,and focuses on the molecular typing methods most used in epidemiologic studies and infection control.These molecular typing methods can be categorized into two main groups:①protein based methods,for example,mutilocus enzyme electrophoresis and immunoblot and ②DNA based methods such as plasmid analysis、restriction endonuclease analysis of chromosomal DNA、 ribotyping、pulsedfield gel electrophoresis and PCR.Finally,this article illustrates some applications of molecular typing in epidemiologic studies and infection control by examples.
作者 陈卫东 李丽
出处 《陕西医学检验》 CAS 2000年第3期11-13,共3页 Shaanxi Journal of Medical Laboratory Sciences
关键词 医院内感染 流行病学分型 抗菌药物敏感性 分子技术 Nosocomial infections Epidemiologic typing Antimicrobial susceptibility Molecular techniques
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参考文献7

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同被引文献23

  • 1李燕俊,刘秀梅.脉冲场凝胶电泳技术及其在致病菌研究中的应用[J].国外医学(卫生学分册),2005,32(5):305-309. 被引量:18
  • 2李铁华,刘小倩.脉冲电场凝胶电泳的原理及在微生物研究中的应用[J].中国地方病防治,1996,11(6):354-355. 被引量:3
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