摘要
目的：建立测定人血浆中阿那曲唑药物浓度的ＧＣ－ＥＣＤ方法。方法：以安定为内标，用乙醚一次提取，ＨＰ－５０毛细管柱测定。结果：检测浓度在０．５－２０μｇ·Ｌ－１内，线性方程为Ｙ＝０．０１７８ｃ－００２８４，ｒ＝０．９９９８。方法回收率平均为９７．５４％，３种浓度１．０，１０．０，２０．０μｇ·Ｌ－１提取回收率分别为８４．５４％，９３．９４％，９７．０３％；日内、日间精密度分别为４．７９％，２．１３％，１．７１％及６．７０％，２．９６％，４．１７％（ｎ＝５），在志愿者单剂量口服阿那曲唑１ｍｇ后，０．３３－１０６ｈ内血药浓度范围在１．２７－１０．１９μｇ·Ｌ－１。结论：该法简便、准确、灵敏，可用于测定该药在人血浆中的药物浓度。
OBJECTIVE: To establish a GC-ECD method to determine the concentration of anastrozole in human plasma. METHODS: Using diazepam as internal standard. Plasma samples were extracted singly with 7ml of ether, and determined with HP-50+ capillary column. RESULTS: The regression equation of anastrozole was y=0.0178c-0.0284, r=0.9998 in the concentration range from 0.5-20μg·L-1.The average relative recovery was 97.54%. Anastrozole standard concentrations were 1 .0, 10.0, 20.0 μg.L-1, the relative standard deviation (RSD) of within-day were 4.79 %, 2. 13 %, and 1 .71 %, the RSD of between-day were 6.70 %, 2.96 %, 4. 17 %, the extraction recovery were 84.54%, 93.94%, 97.03%, respectively. One volunteer was given orally a single ling anastrozole, the range of the plasma concentrations was betwteen 1 .27 μg·L-1 and 10. 19 μg·L-1 at 0.33-106h. CONCLUSION: The method is accurate, sensitive and rapid. It is applicable to determine the concentration of anastrozole in human plasma.
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2001年第2期143-145,共3页
The Chinese Journal of Clinical Pharmacology
