摘要
目的获取人幽门螺杆菌 (helicobacter pylori,Hp)热休克蛋白 A基因 (hsp A )进行核苷酸序列分析。方法利用 PCR技术扩增 Hsp A的 DNA,并将其定向插入 Pin Point TMXa- 3载体中进行核苷酸序列分析。结果所克隆的 Hsp A DNA序列与 Gene Bank公布的序列有 15个碱基存在差异 ,同源 95 .8%。结论所克隆的 Hsp A基因可用于重组表达及相关研究。
ObjectiveTo obtain DNA of human Helicobacter pylori (Hp) heat shock protein A(hspA), and investigate the nucleotide sequence. MethodsThe HspA DNA was amplified by PCR from the chromosomal DNA of clinical isolated Helicobacter pylori and inserted into PinPoint TM Xa-3 Then the sequence was analysed. ResultsThe HspA gene's nucleotide homology was 95 8% as compared to that in Genebank. ConclusionA confirmed HspA gene has been obtained, thus a good foundation for recombination, expression and associated research laid. [
出处
《免疫学杂志》
CAS
CSCD
北大核心
2001年第3期185-188,共4页
Immunological Journal
基金
国家"九五"重点攻关课题! (96 - 90 1- 0 1- 5 4)
全军"九五"医药卫生科研基金! (98D0 0 4)资助项目
