摘要
目的 探讨TNFα和IL 1在皮肤撕脱伤后早期继发血栓形成的作用。方法 在猪后肢形成 12cm× 4cm撕脱皮瓣 ,不同时间取皮肤并抽取静脉血 ,采用双抗夹心ELISA技术和3[H] TdR掺入法 ,检测血浆和皮肤中TNFα的含量和血清IL 1活性。结果 血浆和皮肤中TNFα含量明显升高 (P <0 .0 1) ,3d分别为 (415± 2 4)、(2 98± 18.5 )ng·L-1;血清中IL 1的活性 3d时也增高明显 (P<0 .0 5 ) ,为 (2 .5 9± 0 .85 )。结论 血浆、皮肤内TNFα和血清IL 1含量和活性变化在诱导。
Objective To study the role of TNFα in the plasma and skin and IL 1 in the serum in the formation of secondary thrombosis after skin avulsion. Methods After avulsive flap at size of 12 cm×4 cm was inflicted on the hind legs of pigs, skin specimens and venous blood samples were taken at various time points. The contents of TNFα in plasma and skin were determined with radio immunoassay, and the activity of serum IL 1 with 3[H] TdR. Results The TNFα contents in the plasma and skin were increased significantly after avulsion( P <0.01),which were (415±24) ng/L and (298±18.5) ng/L respectively on the 3rd day after the injury. The activity of IL 1 in the serum was increased ( P <0.05) and was (2.59 ±0.85) ng/L on day 3. Conclusion The changes of TNFα contents and IL 1 activity in blood and skin play important roles in the inducetion and aggravation of secondary tissue necrosis and early thrombosis after skin avulsion.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2001年第5期562-564,共3页
Journal of Third Military Medical University
基金
国家自然科学基金资助项目! ( 394 70 70 5)
