摘要
以宁夏吴忠胡萝卜、陕西华县胡萝卜和陕西汉中胡萝卜 3个地方品种为材料 ,用PCR (polymerasechainreaction)的方法克隆了中国胡萝卜的抗冻蛋白基因 (afp) ,测定了其核苷酸序列 ,并和英国胡萝卜的afp序列进行了对比。在所测 10 0 4个核苷酸中 ,两变种碱基不同者有 36个 ,占 3.6 % ,其中无义突变 2 1个 ,有义突变 15个。按有义突变计 ,同源性为98.5%。
Three carrot cultivar Wuzhong Ningxia,Huaxian Shaanxi and Hanzhong Shaanxi were used as test material and antifreeze protein gene( afp )of Chinese carrot( Daucus carota var. sativus Hoffm Deutschl)was cloned and sequenced by PCR(polymerase chain reaction).Obtained sequence was compared with that of British carrot( Daucus carota var. autumn King).There were 36 different bases in 1004 nucleotides(3.6%)between the two va rieties. Among the different bases there were 21 nonsense mutations and 15 sense mutations.According to sense mutations,homology was 98.5%.
出处
《园艺学报》
CAS
CSCD
北大核心
2001年第2期173-174,共2页
Acta Horticulturae Sinica
基金
陕西省攻关项目!〔98K12 G19( 11)〕
关键词
胡萝卜
抗冻蛋白
分子生物学
基因克隆
Carrot( Daucus carota var. sativus Hoffm Deutschl)
Antifreeze protein
Molecular biology
