摘要
目的 探索体外培养扩增成年及老年动物室管膜下区 (SVZ)神经干细胞的实用方法。方法 取 8、14及 2 4三个月龄SD大鼠SVZ组织块置含bFGF的DMEM/F12 +B2 7培养液培养 ,Nestin免疫组化法鉴定细胞表型。结果 各月龄的SVZ组织块均能长出Nestin阳性的神经小球及神经干细胞 ,培养 7～ 10天产生的神经球最多 ,神经干细胞状态最佳。结论 用含bFGF的培养基培养成年和老年大鼠SVZ组织块能产生较多的神经干细胞 ;此法是一种具有实用价值的培养、扩增神经干细胞的手段。
Objective To explore a useful method of explant cultures of stem cells in subventricular zone(SVZ) of adult and aged rats. Method The explant slices containing SVZ of rats(8,14 and 24 months old) were in vitro cultured in the medium of DMEM/F 12 +B 27 with bFGF(20ng/ml). The phenotype of cultured cells was identified by immunocytochemistry for nestin. Results Nestin positive neural stem cells and neurospheres could be grown from all SVZ explant cultures. During the period of 7 to 10days in vitro,the number of growing neurospheres was largest and the conditions of neural stem cells were best. Conclusion Many neural stem cells can be obtained from culturing explants containing SVZ derived from adult and aged rats. The explant culture is a valuable method of culturing neural stem cells.
出处
《解剖学研究》
CAS
2001年第1期50-51,共2页
Anatomy Research
